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KMID : 1140120070120020079
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Cancer Prevention Research
2007 Volume.12 No. 2 p.79 ~ p.83
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Optimization of HCV Replicon System for the Study on Hepatocellular Carcinoma
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Kim Ki-Yoon
Seo Young-Rok
Choe Won-Chae
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Abstract
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Hepatitis C virus (HCV) is the major cause of non-A, non-B hepatitis, which often leads to liver cirrhosis and hepatocellular carcinoma. More than 170 million individuals worldwide are infected with HCV. HCV is an enveloped virus with a single-stranded, Linear, positive sense RNA genome approximately 9.5 kb in length. It contains ORF capable of encoding a polyprotein precursor of 3,011 amino acids. There is non-cording region (NCR) or 324-341 nucleotides at the 5’ end and a 3’ NCR of variable length including a poly(U) tract. The 5’ NCR contains an IRES apparently similar in function
(but non structure) to that of picornaviruses Hepatitis C virus (HCV) is a positive-stranded RNA virus that causes severe liver diseases, such as cirrhosis and hepatocellular carcinoma. Propagation of HCV in cell culture has for a long time not been possible because different technical developments. Replicons are molecules that are capable of self-amplification. In this report, we show the optimization for the establishment of the stable HCV replicon system. Also, possible approaches to treatment of Hepatocellular carcinoma using the HCV replicon system are discussed. (Cancer Prev Res 12, 79-83, 2007)
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KEYWORD
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HCV, Hepatocellular carcinoma, HCV replicon system
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